Real-time microarray platform
HybLive™ is an innovative real-time microarray instrument that integrates hybridization, active mixing, temperature control and a live fluorescence microarray imaging into a compact benchtop package.
HybLive™ allows to perform a variety of experiments unachievable with conventional biochip tools, such as quick multiplex on-chip melting curve determination, reliable genotyping, on-chip protein interaction studies, optimization of hybridization conditions...
HybLive™ will help you discover new frontiers in microarray applications while saving optimization and process time.
HybLive™ is a microarray platform for Research Use only.
Associated products
More information
Benefits
- Open platform compatible with standard microscope slide format
- Real-time monitoring of experiment
- Walk-away operation mode
- Excellent accuracy of quantification
- Enhanced specificity
Key Features
- All low and high density arrays compatibles
- 10µm optical resultion
- Dye compatibility: Cy®3/Cy5
- Small footprint with large capabilities
- Dedicated control and analysis software
Applications
- Multiplexed on-chip melting curve measurement
- Genotyping, SNP detection, MLST
- DNA and protein arrays
- Ultrafast probe verification and validation
- Quick optimization of hybridization and washing conditions
- Antibody specificity determination.
Application Notes (for research use only):
Easy on-chip melting curve measurement
The graphs present normalized melting profile and temperature derivative for 11 pairs of perfect matched (PM) - mismatched probes (MM). The PM probes are in blue and MM probes in red. Melting curves are measured by applying a temperature ramp to the hybridized microarray and recording the fluorescence signal decrease as a function of temperature. For each probe, the background-substracted signal is normalized against its value before heating and further normalized against a reference probe. The resulting melting curve is fitted with two parameters and the first derivative is taken to calculate the respective melting temperatures.
Acquisition and analysis software
HybLive™ comes with a dedicated control and analysis matching software package. It has been designed with versatility and ease-of-use in mind, and the result is a powerful tool that integrates perfectly with the hardware.
Programming an experiment is a matter of seconds thanks to the hybridization and washing protocol templates included? Any parameter and setting can be overridden, should you need finer control over the hybridization process than these templates provide.
Detailed and robust analysis of the sequence of images is easily performed. The results can be plotted on a variety of graphs and exported in various standard formats.
Specifications
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Number of colors | 2* |
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Compatible fluorophores | Cy3/Cy5 and analogous* |
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Light source | Solid state |
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Sensor | High quantum efficiency cooled CCD |
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Optical resolution | 10µm |
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Frame rate | Up to 10 images per minute |
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Image size | 2.2 Mpixels (full resolution) |
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Image depth | 16 bits |
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Number of wash solutions | 6 |
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Temperature control accuracy | +/-0.1°C |
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Temperature range | 10 to 85°C |
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Mixing | Highly efficient active mixing technology |
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Dimensions | L45xD50xH55cm |
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Weight | 35kg |
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Cartridge & Slides | |
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Cartridge | Reusable and compatible with standard format slides |
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Recommended Microarray slide | AmpliSlide™ ** |
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Spotting area | 13.5x13.5mm *** |
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Maximum spot quantity | 10k (at 125µm pitch) **** |
* Can be customized up to 4 colors. Compatible fluorophores and light source change accordingly.
** AmpliSlide™ is Genewave's high sensitivity microarray slide (25x75x1.0mm). Please contact us to obtain guidelines for using HybLive™ system with other type of microarray slides.
*** The arrayed slide can be easily assembled to the cartridge's body prior to use. Please contact us for enquiries about arraying solutions.
**** Please contact us for arrraying with higher density.
