Benefits

• Perfect choice for Genewave slides
• Convenient for both automatic and manual protocols
• High quality spot morphology
• High spot-to-spot reproducibility
• Reduced experiment time using premixed solutions
• Improved performance of microarray experiments

Spotting Buffers

These reagents are optimised for spotting of cDNA molecules, PCR products and oligonucleotides (long and short). Chemical components contained in these ready-to-use buffers enhance the immobilisation of samples and the morphology of spots.

Three different spotting buffers are available:

• Spot A: for aminosilane slides;
• Spot E: phosphate-based for epoxysilane slides;
• Spot LE: Low Evaporation for epoxysilane slides.

Blocking Buffer

This blocking buffer reacts rapidly with residual binding site of the slide surface in order to prevent non-specific and undesirable coupling of the fluorescent target to the printed and unprinted areas of the microarray. This leads to more specific distribution of sample and reduced background in the signals.

Hybridization Buffers

These ready-to-use formamide-based buffers are compatible with both aminosilane and epoxysilane chemistries. They have been designed to increase hybridization efficiency and kinetics.

Two different hybridization buffers are available:

• Hyb Buffer: for all hybridizations;
• Hyb Plus Buffer: optimised for highly concentrated hybridization samples.

Washing Buffers

Developed to enhance pre and post hybridization processing, these washing buffers help eliminating residual chemicals and biologicals molecules used during the whole microarray manufacturing and that are non specifically adsorbed onto the surface.

Quality Control

• Quality control for concentration, absorbance and pH values
• High purification by microfiltration
• Produced under standardised conditions

Storage Conditions

• Store at room temperature
• 6 months shelf-life after opening or under sterile conditions

Specifications

* Recommended for highly concentrated samples
** High Stringency Solution

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